Evaluation of the in Vitro Effect of Glyphosate-based Herbicide on Bovine Lymphocytes Using Chromosome Painting

The induction of bovine chromosome 1 aberrations was investigated in cultivated peripheral lymphocytes of cattle after an application of a glyphosate-based herbicide formulation. Fluorescence in situ hybridization with bovine chromosome 1-specific painting probe was used for metaphase cell examination. The cultures were exposed to herbicide at concentration levels, ranging from 28 to 1 120 μmol/L for the last 24 h. Structural chromosome aberrations of chromosome 1 (acentric fragments) were detected, but without a statistical significance. Slight bovine chromosome 1 aneuploidy increase (monosomy, trisomy) was found in cultures after 24 h treatment with a dose of 56 μmol/L. Statistically significant elevation in polyploidy induction was shown at the same concentration (P<0.05).